Neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS) is localized in the chromosomal region 1p13.2. It is a part of the RAS gene family which encode proteins involved in signal transmission in cells and participate in the regulation of cell growth. It plays a central role in MAPK signaling pathway. Point mutations of the NRAS gene have been found in a variety of tumor types, e.g. melanoma (13-25%), colorectal cancer (1-6%), lung cancer (1%), hepatocellular carcinoma (10%), thyroid carcinomas (7 %), etc.
We perform the examination of the NRAS gene with a focus on the detection of activating mutations in codons 12, 13, 59, 60, 61, and 146.
We use PCR and reverse hybridisation using NRAS XL StripAssay (ViennaLab) kit.
Analytical sensitivity and specificity of the sequencing: 99%.
In the case of the analysis of somatic mutations the mutations will not be detected, if the altered cell line is not represented by at least 20% (sequencing), or 1% (reverse hybridization).
We also perform PCR and reverse hybridization analysis using NRAS StripAssay (ViennaLab).
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